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HOME > Protocols > Media and Reagents > M9 Minimal Media Recipe (1000 ml)

M9 Minimal Media Recipe (1000 ml)

  1. Make M9 salts
  2. To make M9 Salts aliquot 800ml H2O and add
      • 64g Na2HPO4-7H2O
      • 15g KH2PO4
      • 2.5g NaCl
      • 5.0g NH4Cl
      • Stir until dissolved
      • Adjust to 1000ml with distilled H2O
      • Sterilize by autoclaving
  3. Measure ~700ml of distilled H2O (sterile)
  4. Add 200ml of M9 salts
  5. Add 2ml of 1M MgSO4 (sterile)
  6. Add 20 ml of 20% glucose (or other carbon source)
  7. Add 100ul of 1M CaCl2 (sterile)
  8. Adjust to 1000ml with distilled H2O

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