M9 Minimal Media Recipe (1000 ml)

1. Make M9 salts
2. To make M9 Salts aliquot 800ml H₂O and add
   • 64g Na₂HPO₄-7H₂O
   • 15g KH₂PO₄
   • 2.5g NaCl
   • 5.0g NH₄Cl
   • Stir until dissolved
   • Adjust to 1000ml with distilled H₂O
   • Sterilize by autoclaving
3. Measure ~700ml of distilled H₂O (sterile)
4. Add 200ml of M9 salts
5. Add 2ml of 1M MgSO₄ (sterile)
6. Add 20 ml of 20% glucose (or other carbon source)
7. Add 100ul of 1M CaCl₂ (sterile)
8. Adjust to 1000ml with distilled H₂O