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HOME > Protocols > Media and Reagents > Recipe for 2% Paraformaldehyde

Recipe for 2% Paraformaldehyde

To make 100ml of 2% Paraformaldehyde

  1. Add 2 grams of paraformaldehyde to 48 ml of water
  2. Heat to dissolve
  3. Add NaOH dropwise until solution clears (10-20 drops of 2M)
  4. Add 50ml of 2x PBS and mix
  5. Remove from heat and place on ice
  6. pH from 7.2 to 7.4

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