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HOME > Protocols > Media and Reagents > Marine Broth Recipe

Marine Broth Recipe

    1. Dissolve the following in 850ml of distilled water
        • 5g Bacto peptone
        • 1g Yeast extract
        • 0.1g Fe(III) citrate
        • 19.45g NaCl
        • 5.9g MgCl2 (anhydrous)
        • 3.24g NaSO4
        • 1.8g CaCl2
        • 0.55g KCl
        • 0.16g Na2CO3
        • 0.08g KBr
        • 0.034g SrCl2
        • 0.022g H3BO3
        • 0.004g Na-silicate
        • 0.0024g NaF
        • 0.0016g NH4NO3
        • 0.008g Na2HPO4
    2. Adjust pH to 7.6
    3. Bring to 1000ml with distilled water
    4. Autoclave or filter sterilize

Related links: Marine Agar Recipe


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