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- Combine the following in 800ml water:
- 3g CaCO3
- 20mg Bromocresol green
- 0.1ml Tween 80
- 0.56g MnSO4 x H2O
- 1.025g MgSO4 x 7 H2O
- 5g NaCl
- 5g Yeast extract
- 10g Bacto peptone
- 10g Maltose
- 40g Saccharose
- Adjust to 1000ml with distilled water
- Autoclave to sterilize.
Related pages: Saccharose agar recipe
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