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HOME > Protocols > Media and Reagents > Wolfe's Mineral Solution Recipe

Wolfe's Mineral Solution Recipe

  1. Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve.
  2. Add the following:
    • 3.0g MgSO4-7H2O
      0.5g MnSO4-H2
      1.0g NaCl
      0.1g FeSO4-7H2O
      0.1g CoCl2-6H2O
      0.1g CaCl2
      0.1g ZnSO4-7H2O
      0.01g CuSO4-5H2O
      0.01g AlK(SO)4-12H2O
      0.01g H3BO3
      0.01g Na2MoO4-2H2O
  3. q.s. to 1000ml
  4. Filter sterilize

Related reagents: Wolfe's Vitamin Solution Recipe

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