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HOME > Protocols > Media and Reagents > Wolfe's Vitamin Solution

Wolfe's Vitamin Solution Recipe

  1. Dissolve (or add approriate concentrations solutions) for the following into 900ml of distilled water
    • 10mg Pyridoxine hydrochloride
    • 5.0mg Thiamine-HCl
    • 5.0mg Riboflavin
    • 5.0mg Nicotinic acid
    • 5.0mg Calcium D-(+)-pantothenate
    • 5.0mg p-Aminobenzoic acid
    • 5.0mg Thioctic acid
    • 2.0mg Biotin
    • 2.0mg Folic Acid
    • 0.1mg Vitamin B12
  2. q.s. to 1000ml
  3. Filter sterilize

Related reagents: Wolfe's Mineral Solution Recipe


 
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