Bang's reagent for glucose determination

1. Dissolve 100g of K₂CO₃ in 700ml of H₂O at 30^oC
2. Dissolve 66g of KCl in the solution from step 1
3. Dissolve 160g KHCO₃ in the solution from step 2. Please note that the sequence these are added is important
4. Add 4.4g CuSO₄ to the solution from step 3
5. q.s. to 1 liter after CO₂ release
6. After 24 hours, 300ml are diluted to 1 liter using saturated KCl and the resulting solution used after 24 hours. 50ml is equivalent to 10mg glucose

Caution: This solution should always be shaken in a manner to not allow entry of air. This is especially critical after the addition of the CuSO₄

Related reagents: Benedict's solution