Recipe for Benedict's solution for glucose estimation

1. Heat 800ml H₂O.
2. Add 173g of sodium citrate and 100g of Na₃CO₃.
3. q.s to 850ml with H₂O.
4. Separately dissolve 17.3g of CuSO₄.5H₂O in 100ml H2O.
5. Slowly pour the solution from step into the solution from step 3 with constant stirring.
6. q.s. to 1000ml with H₂O.

Related reagents: Bang's Reagent