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HOME > Protocols > Histology and Immunohistochemistry > Staining protocols and reagents
Staining Protocols and Reagents

Staining Protocols
B-gal staining with colloidal gold
- B-gal staining of tissues with colloidal gold antibodies.
CAT staining of frozen sections
- Chloramphenicol acetyltransferase staining of tissue.
IHC of CD80 and CD86
- Immunohistochemistry staining of CD80 and CD86 in tissues.
IHC of hematopoetic cells
- Immunohistochemistry staining of hematopoetic cells in tissues.
Immunofluorescence Staining of frozen tissues
- Generic protocol for immunofluorescence staining of frozen tissue sections.
Immunofluorescence staining of parafin tissues
- Generic protocol for immunofluorescence staining of paraffin tissue sections.
Immunoperoxidase staining of frozen tissues
- Generic protocol for immunoperoxidase staining of frozen tissue sections.
Immunoperoxidase staining of parafin tissue sections - Generic protocol for immunoperoxidase staining of parafin tissue sections.
X-gal staining - Protocol for x-gal staining of lung tissue.

Staining Reagents
Acetic Lacmoid - Acetic lacmoid stain Recipe.
Acetic Orcein - Acetic orcein stain Recipe.
Carmalum stain - used for cell nuclei.
Giemsa stain - used on blood and bone marrow.
Mallory's Triple Stain - A reagent especially suitable for studying connective tissue.
Masson's stain - a trichrome stain for connective tissue.
Methyl Green - a commonly used counterstain that stains cell nuclei light green.
Schiff's reagent - structures rich in polysaccharides, mucopolysaccharides, or glycolipids are the principal tissues stained with Schiff's reagent after acid degreadation.

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